Tuesday, July 29, 2008

God Smiles When We Use Our Abilities by Rick Warren

He has shaped each person in turn; now he watches everything we do. Psalm 33:15 (MSG)

*** *** *** ***

After the flood, God gave Noah these simple instructions: ''Be fruitful and increase in number and fill the earth. Everything that lives and moves will be food for you. Just as I gave you the green plants, I now give you everything" (Genesis 9:1, 3 NIV).

God said, "It's time to get on with your life! Do the things I designed humans to do. Make love to your spouse. Have babies. Raise families. Plant crops and eat meals. Be humans! This is what I made you to be!"

You may feel that the only time God is pleased with you is when you're doing "spiritual" activities like reading the Bible, attending church, praying, or sharing your faith. And you may think God is unconcerned about the other parts of your life. Actually, God enjoys watching every detail of your life, whether you are working, playing, resting, or eating. He doesn't miss a single move you make. The Bible tells us, "The steps of the godly are directed by the Lord. He delights in every detail of their lives" (Psalm 37:23 NLT).

Every human activity, except sin, can be done for God's pleasure if you do it with the attitude of praise. You can wash dishes, repair a machine, sell a product, write a computer program, grow a crop, and raise a family for the glory of God. Like a proud parent, God especially enjoys watching you use the talents and abilities he has given you. God intentionally gifted us differently for his enjoyment. He has made some to be athletic and some to be analytical. You may be gifted at mechanics or mathematics or music or a thousand other skills. All of these activities can bring a smile to God's face.

You don't bring glory or pleasure to God by hiding your abilities or by trying to be someone else. You only bring him enjoyment by being you. Anytime you reject any part of yourself, you are rejecting God's wisdom and sovereignty in creating you. God says, "You have no right to argue with your Creator. You are merely a clay pot shaped by a potter. The clay doesn't ask, 'Why did you make me this way?'" (Isaiah 45:9 CEV).

In the film Chariots of Fire, Olympic runner Eric Liddell says, "I believe God made me for a purpose, but he also made me fast, and when I run, I feel God's pleasure." Later he says, "To give up running would be to hold him in contempt." There are no unspiritual abilities, just misused ones. Start using yours for God's pleasure.

God also gains pleasure in watching you enjoy his creation. He gave you eyes to enjoy beauty, ears to enjoy sounds and music, your nose and taste buds to enjoy smells and tastes, and the nerves under your skin to enjoy touch. Every act of enjoyment becomes an act of worship when you thank God for it. In fact, the Bible says, "God generously gives us everything for our enjoyment" (1 Timothy 6:17 TEV).

God even enjoys watching you sleep! When my children were small, I remember the deep satisfaction of watching them sleep. Sometimes the day had been filled with problems and disobedience, but asleep they looked contented, secure, and peaceful, and I was reminded of how much I loved them.

My children didn't have to do anything for me to enjoy them. I was happy to just watch them breathing. As their little chests would rise and fall, I'd smile, and sometimes tears of joy filled my eyes. When you are sleeping, God gazes at you with love, because you were his idea. He loves each one of us as if there were only one of us.

Parents do not require their children to be perfect, or even mature, in order to enjoy them. They enjoy them at every stage of development. In the same way, God doesn't wait for you to reach maturity before he starts liking you. He loves and enjoys you at every stage of your spiritual development.

You may have had unpleasable teachers or parents as you were growing up. Please don't assume God feels that way about you. He knows you are incapable of being perfect or sinless (1 John 1:8). The Bible says, "He certainly knows what we are made of. He bears in mind that we are dust" (Psalm 103:14 GW).

What God looks at is the attitude of your heart: Is pleasing him your deepest desire? This was Paul's life goal: "More than anything else, however, we want to please him, whether in our home here or there" (2 Corinthians 5:9 TEV).

When you live in light of eternity, your focus changes from "How much pleasure am I getting out of life?" to "How much pleasure is God getting out of my life?"

God is looking for people like Noah in the 21st century – people willing to live for the pleasure of God. The Bible says, "The Lord looks down from heaven on all mankind to see if there are any who are wise, who want to please God" (Psalm 14:2 LB).

Will you make pleasing God the goal of your life? There is nothing that God won't do for the person totally absorbed with this goal.

Monday, July 7, 2008

Yosh~~~

Haha... Hi guys... So sorry that I have to take such a long time to put up my own blog... Mainly the reason is to compile as many story as possible before uploading them for ur reading so that u only need to read my blog once in awhile, and of coz for easier maintenance also... Hope you enjoy reading my blogs and have a blessed day ahead of you~~~ God bless U~~!

Master degree: Ups and Downs...

Weird... Felt like writing yet again the exciting stuffs that happened to me during my RA and master degree period. Learnt a lot of stuff during this period of time. I had chosen to write out the things I learnt as I remembered them.

After the acceptance by my supervisors, I got busy with the preparations of proposal. And of course the application forms were abit of problems as my referee reports got lost even I sent them in end of July by my referees' courtesy. That aside, coming up with a proposal title proved to be a headache. I am glad that my supervisors chose to let me do things on my own just after giving me slightest idea about what is expected. Prof. Sim gave me five (5) snakes to choose upon for my research. They are (i) Malaysian pit viper; (ii) Malayan cobra; (iii) Malayan krait; (iv) king cobra; and (v) Malaysian lance-headed pit viper. I chose Malayan krait as my snake and hand in the proposal for first review.

After both supervisors discussed among themselves regarding my proposal, it was concluded that the snake proposed wasn't the best choice. Malayan cobra seems to be a better choice as the venom composed of three major components: (i) PLA2; (ii) neurotoxin; and (iii) cardiotoxin. In short, this is the perfect snake as the venom has different mode of action. Sufficient for my studies. We have tonnes of the venom in the lab, readily available.

After a few more reviews from both of them, my proposal is completed. This is the period where my Aax2 will be having her convocation. After her convocation, we went back to Sandakan together. Shortly after that my grandma passed, if not mistaken at 7.35am on 5th of October 2007, Friday where it is the last day of PMR. Thank God that I was back home where I am helping out in handling some of the logistic matters. After the burial on the 10th or 11th, my mom and younger brother stayed back a week longer as my bro was having after PMR break and I believed my mom wouldn't want to return home after awhile. I decided to spend my time with them until they leave KL. Thank God that both my supervisors let me do so and it was then that I realised all of them in the lab (except for two Malay lab assistants) are Christians! Amazing! It was then that I finally realized that I am in good hands.

I got along with them very well, except for the PHD student and PRof. Tan as I don't see them that often. Shin Yee was having her annual break and was expecting a child soon. Therefore I supposed the bossy-instructing attitude could be mainly due to the hormonal issue. Prof Tan in the meantime always "sigh" in his office. Thus I keep my distance from him, afraid that I add on to his burden in anyway. Nevertheless, they are pleasant people to get along with by the way they interact with others.

Kak Shikin taught me the first few days to do SDS-PAGE and Western Blotting using Invitrogen Western Blot Kit. The SDS-PAGE kit was quite difficult to master as it always leak. Through many practices and leaving it overnight are the keys to ensure correct setup. I had be repeating SDS-PAGE and Western Blot for about 6 times in about a month. It was then the chromogen from the kit expired and thus the work stopped until now. The blotting stuff hadn't arrive yet tough the chromogens arrived in January. I did also column chromatography during this period of time. The velvet bean extracts was ran through the Con-A Sepharose column to isolate the glycoprotein of interest, which shows off as a peak in elution buffer tube 7 onwards by Bradford assays. Again, Bradford assays were repeated over and over again to finally get a reliable result. I noticed that there are two ways to prepare the Bradford solution.

There are three ingredients needed in order to prepare the Bradford solution:
(i) 100ml of 85% (v/v) phosphoric acid
(ii) 100mg of Coomassie Brilliant Blue G
(iii) 50ml of 95% ethanol

The instructions to prepare them is simple: 100ml of 85% (v/v) phosphoric acid was added to 100mg of Coomassie Brilliant Blue G and dissolved in 50ml 95% ethanol, followed by topping it up to final volume of 1 liter. One of the lab assistant followed this method and the resulting solution is dark blue in colour. I noticed that when we use this method to prepare the BRadford solution, the powder took a very long time to dissolve completely. I wonder whether we should add everything up then only stir to dissolve. The other lab assistant however, mixed the two solution first before adding the powder. In this case, the powder dissolved easier and the resulting solution is more black than blue in colour. The first method of preparation gave fluctuating results whereas the second method gave a more stable and consistent results. I read about Bradford assay and most of the literature confirmed that the first method of preparation to be correct. Then where did it go wrong? Beats me... Given the many factors that may influence the results, the time and incubation time are perhaps the variables.

Imagine this: I have 60 samples to be run using Bradford's assay. Each smple requires three replicates. The BSA standard required 33 test tubes alone! Therefore, in total there were about 33+(60*3)=213 test tubes to be read using the spectrophotometer a day... SEGOII... Each test tube are required to be read three times. Gulp** It took me a whole day to read and finish all the samples. I had to prepare the solution a day ahead and kept them in dark.

After getting used to the techniques and time management, slowly better results came into view. Once I asked my Australian senior regarding the preparation of Bradford Solution, he showed me a literature that I came across before. When I asked him whether he faced any complications in preparing the solution, he answered that he didn't know of any. Guess what? He had been using commercially available Bradford solution all this while and never tried bancuh sendiri. Sweatnya >_<" Another alternative to do Bradford assay is by using 96 well microtiter plate. This seems like a better alternative. In this case, a multichannel pipetter can be used and therefore the inconsistency could be minimised greatly. And not to mention that we can also minimised wastage as both the samples and solutions required are greatly reduced. Thinking that I had found the possible solutions, I proposed this alternative to the lab assistant. However, the answer that came back to me was "we never did this in this lab before. We have always used the cuvetter for this all the while... " >~<"

After PRof. Tan was finally satisfied with the results, we moved on to yet another chapter: Building up immunity of rabbits using the MPE fractionated by column chromatography. Of course, this is yet another chapter of my story...

Song: He Will Come and Save You

A song that I appreciated a lot during the times when I am down and lost...

He will come and save you

Intro: G D Em C D G D C2 D (2x)

G D Em C D G D C2 D
Say to those who are fearful hearted, "do not be afraid,
G D Em C D G
the Lord your God is strong, with His mighty arm,
D C2 C D G C2 G G
when you call on His name." He will come and save


G C D G C D Em
He will come and save you, He will come and save you
C D
Say to the weary one, "your God will surely come"
G C D G
He will come and save you

G C D G C D Em
He will come and save you, He will come and save you
C D
Lift up your eyes to Him, you will arise again,
G C D G
He will come and save you


G D Em C D G D C2 D
Say to those who are broken hearted, "do not lose your faith,"
G D Em C D G
the Lord your God is strong, with His loving arms,
D C2 C D G C2 G G
when you call on His name." He will come and save

Em D
He is our refuge in the day of trouble,
G2 Am7
He is our shelter in the time of storm,
G2 C
He is our tower in the day of sorrow,
Am7 D
our fortress in the time of war


(Taken from http://www.higherpraise.com/lyrics1/HeWillComeAndSaveYou.htm)